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ProductsBack/ANPEP / CD13 Antibody (clone B347)

ANPEP / CD13 Antibody (clone B347)

LS-C170936
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
TargetANPEP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANPEP / CD13 Antibody (clone B347)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
  • Applications Supplier
    Flo, IF (5 - 20 µg/ml), IHC (5 - 20 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B347
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID290
  • Target name
    ANPEP
  • Target description
    alanyl aminopeptidase, membrane
  • Target synonyms
    alanyl (membrane) aminopeptidase; aminopeptidase M; aminopeptidase N; AP-M; APN; AP-N; CD13; GP150; hAPN; LAP1; membrane alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; P150; PEPN
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANPEP using anti-ANPEP antibody (A02591-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANPEP antigen affinity purified polyclonal antibody (Catalog # A02591-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANPEP at approximately 150KD. The expected band size for ANPEP is at 150KD.
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Human U937 probed with CD13/APN/ANPEN Polyclonal Antibody (bs-1383R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
CD13 Polyclonal AntibodyBS-1383R
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