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anti-CD13 (human), mAb (22A5) (APC)

ANC-162-060
Ancell Corporation
ApplicationsFlow Cytometry
Product group Antibodies
TargetANPEP
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD13 (human), mAb (22A5) (APC)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    22A5
  • Concentration
    0.5 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID290
  • Target name
    ANPEP
  • Target description
    alanyl aminopeptidase, membrane
  • Target synonyms
    alanyl (membrane) aminopeptidase; aminopeptidase M; aminopeptidase N; AP-M; APN; AP-N; CD13; GP150; hAPN; LAP1; membrane alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; P150; PEPN
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP15144
  • Protein Name
    Aminopeptidase N
  • Scientific Description
    Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. - Monoclonal Antibody. Recognizes the human cell surface aminopeptidase N enzyme [CD13]. Applications: FACS. Isotype: Mouse IgG2akappa. Clone: 22A5. Liquid. 50mM Sodium Phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANPEP using anti-ANPEP antibody (A02591-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANPEP antigen affinity purified polyclonal antibody (Catalog # A02591-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANPEP at approximately 150KD. The expected band size for ANPEP is at 150KD.
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