Anti-3-Phenoxybenzoic acid [3P5ThC12]

Absolute Antibody

Anti-3-Phenoxybenzoic acid [3P5ThC12]

9

The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3-Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfen

The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. The cd-FEIA showed 50% inhibitory concentration (IC50) and the linear range of respectively 0.082 and 0.015-0.447 ng/ml, and a detection limit of 0.011 ng/ml (Huo et al., 2018; PMID: 30293433). The VHH fragment was employed to develop a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-PBA, showing a limit of detection of 3ng/ml (Riangrungro et al. 2019; PMID: 31462650).

ELISA

Research Use Only

Monoclonal

3P5ThC12

Mouse

IgG1

Other Species

-20°C,2°C to 8°C

12352203