Anti-4R-Tau pAb (Rabbit, Antiserum)

Cosmo Bio USA

Anti-4R-Tau pAb (Rabbit, Antiserum)

16

Research Use Only

Anti-4R-Tau pAb - prepared from rabbits immunized with synthetic N279D 4R-tau peptide [VQIIDKKLDLSNVQSKC] - recognizes 4R tau isoforms, regardless of deamidation as occurs extensively in AD brains. Validated for western blot and IHC(p), this isoform-specific tau antibody is useful for immunohistochemical and biochemical studies of tau species in diverse neurodegenerative diseases. Tau is a microtubule-associated protein that assembles into insoluble, filamentous, and hyperphosphorylated inclusions in many neurodegenerative diseases. The adult human brain expresses 6 distinct splice forms divided into two groups depending on the inclusion of exon 10 to generate either 3-repeat (3R) or 4-repeat (4R) isoforms. Distinct tau isoforms accumulate in diseased brains: all 6 isoforms in AD, 3R tau in Picks disease, and 4R tau in PSP and CBD. Therefore, isoform-specific tau antibodies are useful tools for immunohistochemical and biochemical studies of tau species in diseased brains. Tau deposited in AD is extensively deamidated at N279. This anti-4R tau pAb specifically recognizes 4R tau isoforms regardless of deamidation and strongly stained tau in AD brain. Specifications Product type primary antibody Immunogen synthetic N279D 4R-tau peptide (VQIIDKKLDLSNVQSKC) Raised in rabbit Source anti-serum Form liquid with 0.1% NaN3 as a preservative Volume 50 uL Label none Specificity unlabeled 4R-tau isoforms Cross reactivity human, mouse, rat Storage below -20°C. (below -70°C for prolonged storage). Aliquot to avoid cycles of freeze/thaw. Recommended dilutions Western blotting 1/2000-1/5000 Immunohistochemistry 1:3000-1/20000 Other applications have not been tested. Optimal dilutions/concentrations should be determined by the end user. References <ol> Hasegawa M, Watanabe S, Kondo H, et al, 3R and 4R tau isoforms in paired helical filaments in Alzheimers disease. Acta Neuropathol. 2013 Nov 9 Dan A, Takahashi M, Masuda-Suzukake M, et al.Extensive deamidation at asparagine residue 279 accounts for weak immunoreactivity of tau with RD4 antibody in Alzheimers disease brain. Acta Neuropathol </ol>

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