ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
anti-5-Formylcytosines (5-fC), Rabbit Monoclonal (RM477)
REV-31-1369-00
Product group Antibodies
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-5-Formylcytosines (5-fC), Rabbit Monoclonal (RM477)
- Delivery Days Customer10
- CertificationResearch Use Only
- ClonalityMonoclonal
- Scientific Description5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation. - Recombinant Antibody. RM477 reacts to 5-Formylcytosine (5-fC) in DNA. No cross-reactivity with non-modified cytosine and other modified cytosines in DNA. Isotype: Rabbit IgG. Immunogen: KLH-conjugated 5-Formylcytosine. Applications: ELISA, DotBlot. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. 5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203