Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using His-tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 ug). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 ug).