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NCEH1 antibody

FNAB00017
Wuhan Fine Biotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNCEH1
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    anti-AADACL1-antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Estimated Purity
    ≥95%
  • Gene ID57552
  • Target name
    NCEH1
  • Target description
    neutral cholesterol ester hydrolase 1
  • Target synonyms
    AADACL1; acetylalkylglycerol acetylhydrolase; alkylacetylglycerol acetylhydrolase; arylacetamide deacetylase-like 1; NCEH; neutral cholesterol ester hydrolase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6PIU2
  • Protein Name
    Neutral cholesterol ester hydrolase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AADACL1/NCEH1 using anti-AADACL1/NCEH1 antibody (A06478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AADACL1/NCEH1 antigen affinity purified polyclonal antibody (Catalog # A06478-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AADACL1/NCEH1 at approximately 46 kDa. The expected band size for AADACL1/NCEH1 is at 46,55 kDa.
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