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Goat anti-AADACL1

EB08327
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetNCEH1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-AADACL1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID57552
  • Target name
    NCEH1
  • Target description
    neutral cholesterol ester hydrolase 1
  • Target synonyms
    AADACL1; acetylalkylglycerol acetylhydrolase; alkylacetylglycerol acetylhydrolase; arylacetamide deacetylase-like 1; NCEH; neutral cholesterol ester hydrolase 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_065843.3; NP_001139748.2; NP_001139749.1. GeneIDs all Nonhuman: 320024 (mouse);. Purification: Antigen affinity purified. Names and symbols: AADACL1; arylacetamide deacetylase-like 1
  • Reactivity Supplier
    Human, Mouse, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AADACL1/NCEH1 using anti-AADACL1/NCEH1 antibody (A06478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AADACL1/NCEH1 antigen affinity purified polyclonal antibody (Catalog # A06478-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AADACL1/NCEH1 at approximately 46 kDa. The expected band size for AADACL1/NCEH1 is at 46,55 kDa.
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