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Anti-AAMP

Y058093
Applied Biological Materials
ApplicationsWestern Blot, ELISA
Product group Antibodies
100 ul
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-AAMP
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The gene product is an immunoglobulin-type protein. It is found to be expressed strongly in endothelial cells cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a heparin-binding domain and mediates heparin-sensitive cell adhesion.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human AAMP protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (Catalog # PB9123) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAMP at approximately 39 kDa. The expected band size for AAMP is at 39 kDa.
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