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Anti-AASS Antibody

144-62524
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetAASS
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-AASS Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID10157
  • Target name
    AASS
  • Target description
    aminoadipate-semialdehyde synthase
  • Target synonyms
    alpha-aminoadipate semialdehyde synthase; alpha-aminoadipic semialdehyde synthase, mitochondrial; aminoadipic semialdehyde synthase; LKR/SDH; LKRSDH; LORSDH; lysine-2-oxoglutarate reductase; lysine-ketoglutarate reductase /saccharopine dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UDR5
  • Protein Name
    Alpha-aminoadipic semialdehyde synthase, mitochondrial
  • Scientific Description
    AASS pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AASS using anti-AASS antibody (A07302-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AASS antigen affinity purified polyclonal antibody (Catalog # A07302-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AASS at approximately 102 kDa. The expected band size for AASS is at 102 kDa.
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