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Immunohistochemical staining of human liver shows weak to moderate membranous positivity in bile duct cells.
Immunohistochemical staining of human liver shows weak to moderate membranous positivity in bile duct cells.
Immunohistochemical staining of human liver shows weak to moderate membranous positivity in bile duct cells.

Anti-ABCB4 Antibody

HPA053288
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCB4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ABCB4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5244
  • Target name
    ABCB4
  • Target description
    ATP binding cassette subfamily B member 4
  • Target synonyms
    ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3, phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, ATP-binding cassette, sub-family B (MDR/TAP), member 4, P-glycoprotein 3, P-glycoprotein-3/multiple drug resistance-3, multidrug resistance protein 3, multiple drug resistance 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21439
  • Protein Name
    Phosphatidylcholine translocator ABCB4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (PB9275). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PB9275) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 150 kDa. The expected band size for ABCB4 is at 142 kDa.
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