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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Anti-ABCC8 Antibody

HPA042318
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCC8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ABCC8
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6833
  • Target name
    ABCC8
  • Target description
    ATP binding cassette subfamily C member 8
  • Target synonyms
    ABC36; ATP-binding cassette sub-family C member 8; ATP-binding cassette transporter sub-family C member 8; ATP-binding cassette, sub-family C (CFTR/MRP), member 8; HHF1; HI; HRINS; MRP8; PHHI; PNDM3; sulfonylurea receptor (hyperinsulinemia); sulfonylurea receptor 1; SUR; SUR1; SUR1delta2; TNDM2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09428
  • Protein Name
    ATP-binding cassette sub-family C member 8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SUR1 using anti-SUR1 antibody (A00895-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUR1 antigen affinity purified polyclonal antibody (Catalog # A00895-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUR1 at approximately 177KD. The expected band size for SUR1 is at 177KD.
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