Anti-AC toxin [2F5]

Absolute Antibody

Anti-AC toxin [2F5]

7

This antibody is specific for the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of Bordetella pertussis adenylate cyclase toxin.

The mouse version of this antibody was used for immunoblotting on different epitopes of the adenylate cyclase toxin from Bordetella pertussis. It was most sensitive to amino acids 1156-1489 (Lee et al, 1999; pmid:10225859). An ELISA was preformed on purified AC toxin using the mouse version of this antibody. The binding of the mouse version of this antibody was assessed at different calcium concentrations. It showed only a small decrease in binding affinity at high calcium concentration (Hewlett et al, 1991; pmid:1894634).

Western Blot, ELISA

WB; ELISA

Research Use Only

Monoclonal

2F5

Mouse

IgG1

Bacteria

Bordetella pertussis

The mouse version of this antibody was generated by immunizing BALB/c mice with Bordetella pertussis adenylate cyclase toxin purified using sucrose gradient centrifugation.

-20°C,2°C to 8°C

12352203