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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in tubules.

Anti-ACAD9 Antibody

HPA037716
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetACAD9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACAD9
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28976
  • Target name
    ACAD9
  • Target description
    acyl-CoA dehydrogenase family member 9
  • Target synonyms
    acyl-Coenzyme A dehydrogenase family, member 9; complex I assembly factor ACAD9, mitochondrial; MC1DN20; NPD002; very-long-chain acyl-CoA dehydrogenase VLCAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H845
  • Protein Name
    Acyl-CoA dehydrogenase family member 9, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACAD9 using anti-ACAD9 antibody (A05223-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAD9 antigen affinity purified polyclonal antibody (Catalog # A05223-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAD9 at approximately 65 kDa. The expected band size for ACAD9 is at 69 kDa.
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