Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb

PTM BIO

Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb

5

Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the structurally similar crotonylated, hydroxyisobutyrylated, bèta-hydroxybutyrylated, or butyrylated H3 peptide at Lys27 or Lys9. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys9, Lys14, or Lys23.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry Paraffin

WB, IHC-P, ICC/IF, IP, ChIP

Antibody

Research Use Only

Monoclonal

PA-133-28

Unconjugated

Rabbit

IgG

Histone H3.1

Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, Phosphorylatedrylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc.

Stable for 12 months from date of receipt/reconstitution.

Human

Human

Protein A purified

Store at -20°C. Avoid freeze/thaw cycles.

12352203