Anti-Acetylcholinesterase [Elec408]

Absolute Antibody

Anti-Acetylcholinesterase [Elec408]

7

The original antibody (IgG1) bound AChE with high affinity and strongly inhibited the antigen with an apparent Ki value less than 0.1 nM. The relative affinity of the antibody for AChE was evaluated using a competitive radioimmunoassay (Kd= 38 pM). The inhibition of E. electricus AChE by the antibody was studied as a function of substrate concentration, the inhibition obtained with the antibody was about 75 % (Remy et al., 1995; PMID: 7649165). The epitope mapping was studied by site-directed mutagenesis, showing the antibody binds to the AChE back door (Simon et al., 1999; PMID: 10488117). The structure of the Fab fragment was determined by X-ray (Bourne et al., 2013; PMID: 24146971).

Neutralisation/Blocking, Other Application, RadioImmunoAssay

RIA; Crystallization; SPR; Inhibition

Research Use Only

Acetylcholinesterase

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203