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Immunohistochemical staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.

Anti-ACSM3 Antibody

HPA041013
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACSM3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACSM3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6296
  • Target name
    ACSM3
  • Target description
    acyl-CoA synthetase medium chain family member 3
  • Target synonyms
    SA, SAH, acyl-coenzyme A synthetase ACSM3, mitochondrial, SA (rat hypertension-associated) homolog, SA hypertension-associated homolog, butyrate--CoA ligase 3, butyryl-coenzyme A synthetase 3, middle-chain acyl-CoA synthetase 3, propionate--CoA ligase, protein SA homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53FZ2
  • Protein Name
    Acyl-coenzyme A synthetase ACSM3, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SAH/ACSM3 using anti-SAH/ACSM3 antibody (A08748-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAH/ACSM3 antigen affinity purified polyclonal antibody (Catalog # A08748-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAH/ACSM3 at approximately 66 kDa. The expected band size for SAH/ACSM3 is at 66 kDa.
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