Figure 1. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-ACTH/POMC Antibody
PB9039
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetPOMC
Overview
- SupplierBoster Bio
- Product NameAnti-ACTH Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoHistoChemistry
- Applications SupplierIHP, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID5443
- Target namePOMC
- Target descriptionproopiomelanocortin
- Target synonymsACTH; adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; alpha-MSH; beta-endorphin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; CLIP; corticotropin-like intermediary peptide; corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; LPH; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; MSH; NPP; OBAIRH; opiomelanocortin prepropeptide; POC; pro-ACTH-endorphin; pro-opiomelanocortin; proopiomelanocortin preproprotein
- HostRabbit
- IsotypeIgG
- Protein IDP01189
- Protein NamePro-opiomelanocortin
- Scientific DescriptionBoster Bio Anti-ACTH/POMC Antibody Picoband® catalog # PB9039. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityBovine, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203