Figure 1. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-ACTH/POMC Antibody
PB9039-CARRIER-FREE
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetPOMC
Overview
- SupplierBoster Bio
- Product NameAnti-ACTH/POMC Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID5443
- Target namePOMC
- Target descriptionproopiomelanocortin
- Target synonymsACTH, CLIP, LPH, MSH, NPP, OBAIRH, POC, pro-opiomelanocortin, adrenocorticotropic hormone, adrenocorticotropin, alpha-MSH, alpha-melanocyte-stimulating hormone, beta-LPH, beta-MSH, beta-endorphin, beta-melanocyte-stimulating hormone, corticotropin-like intermediary peptide, corticotropin-lipotropin, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, opiomelanocortin prepropeptide, pro-ACTH-endorphin, proopiomelanocortin preproprotein
- HostRabbit
- IsotypeIgG
- Protein IDP01189
- Protein NamePro-opiomelanocortin
- Scientific DescriptionBoster Bio Anti-ACTH/POMC Antibody Picoband® catalog # PB9039. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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