Figure 1. Western blot analysis of Adam28 using anti-Adam28 antibody (A06873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adam28 antigen affinity purified polyclonal antibody (Catalog # A06873-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adam28 at approximately 87KD. The expected band size for Adam28 is at 87KD.
Figure 1. Western blot analysis of Adam28 using anti-Adam28 antibody (A06873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adam28 antigen affinity purified polyclonal antibody (Catalog # A06873-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adam28 at approximately 87KD. The expected band size for Adam28 is at 87KD.
Anti-Adam28 Antibody Picoband(r)
A06873-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetAdam28
Overview
- SupplierBoster Bio
- Product NameAnti-Adam28 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID290344
- Target nameAdam28
- Target descriptionADAM metallopeptidase domain 28
- Target synonymsdisintegrin and metalloproteinase domain-containing protein 28, a disintegrin and metallopeptidase domain 28, a disintegrin and metalloprotease domain 28, a disintegrin and metalloproteinase domain 28
- HostRabbit
- IsotypeIgG
- Protein IDE9PTQ3
- Scientific DescriptionBoster Bio Anti-Adam28 Antibody Picoband® catalog # A06873-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203