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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.

Anti-ADAMTS9 Antibody

HPA028601
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetADAMTS9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ADAMTS9
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56999
  • Target name
    ADAMTS9
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 9
  • Target synonyms
    A disintegrin and metalloproteinase with thrombospondin motifs 9; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P2N4
  • Protein Name
    A disintegrin and metalloproteinase with thrombospondin motifs 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAMTS9 using anti-ADAMTS9 antibody (A04639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat H9C2(2-1) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS9 antigen affinity purified polyclonal antibody (Catalog # A04639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS9 at approximately 216 kDa. The expected band size for ADAMTS9 is at 216 kDa.
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