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Immunohistochemical staining of human liver shows weak cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows weak cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows weak cytoplasmic and nuclear positivity in hepatocytes.

Anti-ADH1A Antibody

HPA060902
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetADH1A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ADH1A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID124
  • Target name
    ADH1A
  • Target description
    alcohol dehydrogenase 1A (class I), alpha polypeptide
  • Target synonyms
    ADH1, alcohol dehydrogenase 1A, ADH, alpha subunit, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase subunit alpha, aldehyde reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07327
  • Protein Name
    Alcohol dehydrogenase 1A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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IHC image of CSB-PA001353LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of ADH1A using anti-ADH1A antibody (PB10029). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates. Lane 4: rat kidney tissue lysates. Lane 5: mouse liver tissue lysates. Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH1A antigen affinity purified polyclonal antibody (Catalog # PB10029) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH1A at approximately 40 kDa. The expected band size for ADH1A is at 40 kDa.
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