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Anti-Adiponectin Receptor 1 Antibody

144-63577
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetADIPOR1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Adiponectin Receptor 1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID51094
  • Target name
    ADIPOR1
  • Target description
    adiponectin receptor 1
  • Target synonyms
    ACDCR1, CGI-45, CGI45, PAQR1, TESBP1A, adiponectin receptor protein 1, progestin and adipoQ receptor family member 1, progestin and adipoQ receptor family member I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96A54
  • Protein Name
    Adiponectin receptor protein 1
  • Scientific Description
    Adiponectin Receptor 1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADIPOR1 antigen affinity purified polyclonal antibody (Catalog # PB9418) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADIPOR1 at approximately 43 kDa. The expected band size for ADIPOR1 is at 43 kDa.
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