Figure 2. Flow Cytometry analysis of A549 cells using anti-ADO antibody (PB10032). Overlay histogram showing A549 cells stained with PB10032 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 2. Flow Cytometry analysis of A549 cells using anti-ADO antibody (PB10032). Overlay histogram showing A549 cells stained with PB10032 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-ADO Antibody Picoband(r)
PB10032-DYLIGHT550
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetADO
Overview
- SupplierBoster Bio
- Product NameAnti-ADO Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 550
- Gene ID84890
- Target nameADO
- Target description2-aminoethanethiol dioxygenase
- Target synonyms2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; C10orf22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); cysteamine dioxygenase
- HostRabbit
- IsotypeIgG
- Protein IDQ96SZ5
- Protein Name2-aminoethanethiol dioxygenase
- Scientific DescriptionBoster Bio Anti-ADO Antibody Picoband® catalog # PB10032. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203