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Immunofluorescent staining of human cell line MCF7 shows localization to cytoplasmic bodies.
Immunofluorescent staining of human cell line MCF7 shows localization to cytoplasmic bodies.
Immunofluorescent staining of human cell line MCF7 shows localization to cytoplasmic bodies.

Anti-AGO1 Antibody

HPA067849
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetAGO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AGO1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26523
  • Target name
    AGO1
  • Target description
    argonaute RISC component 1
  • Target synonyms
    EIF2C, EIF2C1, GERP95, NEDLBAS, Q99, hAgo1, protein argonaute-1, Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, RISC catalytic component, argonaute RISC catalytic component 1, argonaute1, eIF-2C 1, eIF2C 1, eukaryotic translation initiation factor 2C, 1, putative RNA-binding protein Q99
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL18
  • Protein Name
    Protein argonaute-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of AGO1 using anti-AGO1 antibody (A00826-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (Catalog # A00826-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.
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