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Figure 2. Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T450) AKT1 Antibody (A00024T450-1). Immunohistochemistry (IHC) analyzes of p-AKT (T450) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
Figure 2. Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T450) AKT1 Antibody (A00024T450-1). Immunohistochemistry (IHC) analyzes of p-AKT (T450) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
Figure 2. Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T450) AKT1 Antibody (A00024T450-1). Immunohistochemistry (IHC) analyzes of p-AKT (T450) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody

Anti-AKT (phospho-T450) AKT1 Antibody

A00024T450-1
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAKT1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-AKT (phospho-T450) AKT1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    Boster Bio Anti-AKT (phospho-T450) AKT1 Antibody catalog # A00024T450-1. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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