More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-AKT1

Anti-AKT1

Y058027
Applied Biological Materials
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
100 ul
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-AKT1
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biosynth
Akt antibody20R-1920
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-pAKT [AbAb-pAKT]AB04102-10.0
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-AKT1 Antibody144-11016
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Immunofluorescence staining of MCF7 cells using the Anti-AKT1 monoclonal antibody, showing specific staining in the nucleus, cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Product group Antibodies
Atlas Antibodies
Anti-AKT1 AntibodyAMAB90834
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-AKT1,2,3 Antibody Picoband(r)A00024-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613) 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Product group Antibodies
References
GeneTex
AKT1 antibodyGTX110613
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Product group Antibodies
Assay Genie
Phospho-AKT1-S308+AKT2-S309+AKT3-S305 AntibodyCABP1266
ReactivityHuman
TargetAKT1
  • SizePrice
Product group Antibodies
Biomatik
Akt1 Polyclonal AntibodyCAC07008
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Mouse placenta lysates probed with Akt1/3 Polyclonal Antibody, unconjugated (bs-0115M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Product group Antibodies
Bioss
AKT1 Polyclonal AntibodyBS-0115M
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetAKT1
  • SizePrice
Western Blot analysis of A549 cells using Akt Polyclonal Antibody
Product group Antibodies
Cusabio
AKT1/AKT2/AKT3 AntibodyCSB-PA000848
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice