Bio-Connect
IHC analysis of AKT2 using anti-AKT2 antibody (PB9043). AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of AKT2 using anti-AKT2 antibody (PB9043). AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of AKT2 using anti-AKT2 antibody (PB9043). AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-AKT2 Antibody Picoband(r)

Research Use Only
PB9043
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetAKT2
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-AKT2 Picoband Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    WB: The detection limit for AKT2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHP, IHF, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID208
  • Target name
    AKT2
  • Target description
    AKT serine/threonine kinase 2
  • Target synonyms
    HIHGHH; murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; protein kinase Akt-2; protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31751
  • Protein Name
    RAC-beta serine/threonine-protein kinase
  • Scientific Description
    Boster Bio Anti-AKT2 Antibody Picoband® catalog # PB9043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203