IHC analysis of AKT2 using anti-AKT2 antibody (PB9043). AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of AKT2 using anti-AKT2 antibody (PB9043). AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-AKT2 Antibody Picoband(r)
PB9043
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetAKT2
Overview
- SupplierBoster Bio
- Product NameAnti-AKT2 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for AKT2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, IHF, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID208
- Target nameAKT2
- Target descriptionAKT serine/threonine kinase 2
- Target synonymsHIHGHH; murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; protein kinase Akt-2; protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
- HostRabbit
- IsotypeIgG
- Protein IDP31751
- Protein NameRAC-beta serine/threonine-protein kinase
- Scientific DescriptionBoster Bio Anti-AKT2 Antibody Picoband® catalog # PB9043. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203