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ProductsBack/Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody

M00024-5
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetAKT1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17A20
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    Boster Bio Anti-Akt(pan) 1/2/3 Rabbit Monoclonal Antibody catalog # M00024-5. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Distinct roles of TREM2 in central nervous system cancers and peripheral cancers.
    Read this paper
  • Tanshinone IIA Regulates Synaptic Plasticity in Mg(2+)-Free-Induced Epileptic Hippocampal Neurons via the PI3K/Akt Signaling Pathway.
    Read this paper
  • Luteolin overcomes acquired resistance to osimertinib in non-small cell lung cancer cells by targeting the HGF-MET-Akt pathway.
    Read this paper
  • Effects and mechanisms of substance P on the proliferation and angiogenic differentiation of bone marrow mesenchymal stem cells: Bioinformatics and in vitro experiments.
    Read this paper
  • Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation.
    Read this paper
  • Curcumin-enhanced antitumor effects of sorafenib via regulating the metabolism and tumor microenvironment. Man S et al., 2020 Jul 22, Food Funct
    Read this paper
  • Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3alpha Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs. Yang G et al., 2019 Dec 12, Mar Drugs
    Read this paper
  • Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt-SREBP-2 signaling pathway to influence lipid metabolism in HepG2 cells. Xiao J et al., 2019 May, Int J Mol Med
    Read this paper

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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