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Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-ALAS2 Antibody

HPA001638
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetALAS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALAS2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID212
  • Target name
    ALAS2
  • Target description
    5'-aminolevulinate synthase 2
  • Target synonyms
    5-aminolevulinate synthase, erythroid-specific, mitochondrial; 5-aminolevulinic acid synthase 2; ALASE; ALAS-E; aminolevulinate, delta-, synthase 2; ANH1; ASB; delta-ALA synthase 2; delta-ALA synthetase; delta-aminolevulinate synthase 2; SIDBA1; XLDPP; XLEPP; XLSA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22557
  • Protein Name
    5-aminolevulinate synthase, erythroid-specific, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: 5-aminolevulinate synthase, erythroid-specific, mitochondrial antibody at 7ug/ml Lane 1: 293T whole cell lysate Lane 2: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 65, 61, 50, 64 kDa Observed band size: 65 kDa
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Figure 1. Western blot analysis of ALAS2/ASB using anti-ALAS2/ASB antibody (A02525-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALAS2/ASB antigen affinity purified polyclonal antibody (Catalog # A02525-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALAS2/ASB at approximately 65 kDa. The expected band size for ALAS2/ASB is at 65 kDa.
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