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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-ALG3 Antibody

HPA045103
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALG3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALG3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10195
  • Target name
    ALG3
  • Target description
    ALG3 alpha-1,3- mannosyltransferase
  • Target synonyms
    CDG1D, CDGS4, CDGS6, D16Ertd36e, NOT56L, Not56, not, dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase, Not56-like protein, asparagine-linked glycosylation 3 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase), asparagine-linked glycosylation 3 homolog (yeast, alpha-1,3-mannosyltransferase), asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog, asparagine-linked glycosylation protein 3 homolog, carbohydrate deficient glycoprotein syndrome type IV, dol-P-Man-dependent alpha(1-3)-mannosyltransferase, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92685
  • Protein Name
    Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ALG3 using anti-ALG3 antibody (A04688). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALG3 antigen affinity purified polyclonal antibody (Catalog # A04688) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALG3 at approximately 50-55 kDa. The expected band size for ALG3 is at 50 kDa.
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