Anti-ALKBH1 Antibody [JE54-26]

HUABIO

Anti-ALKBH1 Antibody [JE54-26]

2

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

JE54-26

1 mg/ml

Unconjugated

ALKBH1

alkB homolog 1, histone H2A dioxygenase

ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxygenase ALKBH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, alkB, alkylation repair homolog 1, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, alpha-ketoglutarate-dependent dioxygenase ABH1, mRNA N(3)-methylcytidine demethylase, tRNA N1-methyl adenine demethylase

Rabbit

IgG

Nucleic acid dioxygenase ALKBH1

Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Mainly acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs, with a preference for N1-methyladenine at position 58 present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N1-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine to form 5-formylcytosine (f5c) at this position. mt-tRNA(Met) containing the f5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. N6-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing . Demethylates mRNAs containing N3-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation.

-20°C,2°C to 8°C

41116161