Anti-alpha-5 TMD [Nb25]

Absolute Antibody

Anti-alpha-5 TMD [Nb25]

7

Binds to the transmembrane domain of the alpha-5 GABAA receptor. To see the exact epitope see the crystal structure (Miller et al, 2017). Nb25 straddles the neurotransmitter-binding site between adjacent subunits.

The antibody was used to facilitate the crystallisation of the a5 GABAA receptor transmembrane domain where it was shown to form a 1080A2 area interface (approx.). Nb25 was used to increase the soluability and aid packing in the crystal. It has been shown that the binding of Nb25 does not affect the potentiation of the receptor. (Miller et al, 2017)

Other Application

Crystallisation

Research Use Only

Monoclonal

Nb25

GABRA5

gamma-aminobutyric acid type A receptor subunit alpha5

DEE79; EIEE79; GABA(A) receptor subunit alpha-5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid receptor subunit alpha-5; gamma-aminobutyric acid type A receptor alpha5 subunit

Rabbit

IgG

Gamma-aminobutyric acid receptor subunit beta-3

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

A llama was immunized six times with recombinant 1D4-tagged human a1b3 GABAAR pentamer. Nanobodies were selected by phage display, and the clones were then screened for binding to the receptor. From these clones Nb25 was selected.

-20°C,2°C to 8°C

12352203