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Immunohistochemical staining of human liver shows weak cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows weak cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows weak cytoplasmic positivity in hepatocytes.

Anti-AMBP Antibody

HPA001497
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAMBP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AMBP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID259
  • Target name
    AMBP
  • Target description
    alpha-1-microglobulin/bikunin precursor
  • Target synonyms
    A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, protein AMBP, bikunin, complex-forming glycoprotein heterogeneous in charge, growth-inhibiting protein 19, inter-alpha-trypsin inhibitor light chain, protein HC, trypstatin, uristatin, uronic-acid-rich protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02760
  • Protein Name
    Protein AMBP
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Bikunin/AMBP using anti-Bikunin/AMBP antibody (A02419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bikunin/AMBP antigen affinity purified polyclonal antibody (Catalog # A02419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bikunin/AMBP at approximately 34KD. The expected band size for Bikunin/AMBP is at 39KD.
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