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Anti-Amiloride Binding Protein 1 (ABP1) Monoclonal Antibody

CAU36325
Biomatik
ApplicationsFlow Cytometry
Product group Antibodies
TargetAOC1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Anti-Amiloride Binding Protein 1 (ABP1) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26
  • Target name
    AOC1
  • Target description
    amine oxidase copper containing 1
  • Target synonyms
    ABP; ABP1; amiloride binding protein 1 (amine oxidase (copper-containing)); amiloride-binding protein 1; amiloride-sensitive amine oxidase; amiloride-sensitive amine oxidase [copper-containing]; amine oxidase copper domain-containing protein 1; DAO; DAO1; diamine oxidase; histaminase; KAO; kidney amine oxidase
  • Host
    Rabbit
  • Protein IDP19801
  • Protein Name
    Amiloride-sensitive amine oxidase [copper-containing]
  • Scientific Description
    The Anti-Amiloride Binding Protein 1 (ABP1) Monoclonal Antibody (Species: Human) has been validated for the following applications: Flow cytometry.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (A08386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HK-2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody (Catalog # A08386-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABP1/AOC1 at approximately 90 kDa. The expected band size for ABP1/AOC1 is at 85 kDa.
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