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ProductsBack/Anti-Angiogenin [26-2F]

Anti-Angiogenin [26-2F]

AB00400-1.1-BT
Absolute Antibody
ApplicationsELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetANG
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Angiogenin [26-2F]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody binds specifically to angiogenin, a member of of the ribonuclease superfamily of proteins, which interacts with actin on the surface of endothelial and smooth muscle cells, inducing neovascularization. Due to its angiogenic activity, angiogenesis plays a key role in the growth and invasiveness of tumour cells, and therefore neutralising monoclonal antibodies such as 26-2F are important anticancer drugs. The monoclonal antibody 26-2F is not directly cytotoxic to tumour cells in vitro, however has been shown to have antagonistic effects in human breast cancer xenografts in athymic mice.
  • Applications
    ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    26-2F
  • Gene ID283
  • Target name
    ANG
  • Target description
    angiogenin
  • Target synonyms
    ALS9; angiogenin; angiogenin, ribonuclease, RNase A family, 5; epididymis luminal protein 168; HEL168; RAA1; ribonuclease 5; ribonuclease A A1; ribonuclease A family member 5; RNase 5; RNASE4; RNASE5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP03950
  • Protein Name
    Angiogenin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Angiogenin/ANG using anti-Angiogenin/ANG antibody (A00146). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiogenin/ANG antigen affinity purified polyclonal antibody (Catalog # A00146) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiogenin/ANG at approximately 17 kDa. The expected band size for Angiogenin/ANG is at 17 kDa.
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