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Anti-ANXA4 [SAIC-14C-10F12]

AB00308-1.1-BT
Absolute Antibody
ApplicationsMass Spectrometry, Western Blot, ELISA
Product group Antibodies
TargetANXA4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ANXA4 [SAIC-14C-10F12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-14C-10F12
  • Gene ID307
  • Target name
    ANXA4
  • Target description
    annexin A4
  • Target synonyms
    35-beta calcimedin; annexin A4; annexin IV (placental anticoagulant protein II); annexin-4; ANX4; carbohydrate-binding protein p33/p41; chromobindin-4; endonexin I; epididymis secretory protein Li 274; HEL-S-274; lipocortin IV; P32.5; PAP-II; PIG28; placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; protein II; ZAP36
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09525
  • Protein Name
    Annexin A4
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin-4/ANXA4 antigen affinity purified polyclonal antibody (Catalog # A04840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin-4/ANXA4 at approximately 36 kDa. The expected band size for Annexin-4/ANXA4 is at 36 kDa.
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Western blot All lanes: Annexin A4 antibody at 4ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 28 kDa Observed band size: 36 kDa
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