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Immunohistochemical staining of human kidney shows moderate to strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate to strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate to strong membranous positivity in cells in tubules.

Anti-AOC1 Antibody

HPA031033
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetAOC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AOC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26
  • Target name
    AOC1
  • Target description
    amine oxidase copper containing 1
  • Target synonyms
    ABP; ABP1; amiloride binding protein 1 (amine oxidase (copper-containing)); amiloride-binding protein 1; amiloride-sensitive amine oxidase; amiloride-sensitive amine oxidase [copper-containing]; amine oxidase copper domain-containing protein 1; DAO; DAO1; diamine oxidase; histaminase; KAO; kidney amine oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19801
  • Protein Name
    Amiloride-sensitive amine oxidase [copper-containing]
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (A08386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HK-2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody (Catalog # A08386-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABP1/AOC1 at approximately 90 kDa. The expected band size for ABP1/AOC1 is at 85 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA104599(AOC1 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: x200)
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