Anti-Apical membrane antigen 1 [F8.12.19]

Absolute Antibody

Anti-Apical membrane antigen 1 [F8.12.19]

7

This antibody can recognize AMA1 of Plasmodium vivax, Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum in an immunofluorescence assay on mature schizonts. The binding region is a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot. The binding of F8.12.19 to recombinant AMA1 from both P. vivax and P. falciparum was measured by surface plasmon resonance. This antibody shows no function-blocking effect was detected in ex vivo parasite growth inhibition assays. (PMID: 17229439). This antibody a dilution of 1:16,000 was used for the detection of recombinant PvAMA-1 and recombinant PvAMA-DII-III using dot-blot and ELISA assays (PMID: 28855657). This antibody was also used for the identification of PfAMA1 using western blot (PMID: 25236489).

ImmunoFluorescence, Western Blot, ELISA, Other Application

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203