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Anti-APOBEC3A Antibody

HPA043237
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAPOBEC3A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-APOBEC3A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200315
  • Target name
    APOBEC3A
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3A
  • Target synonyms
    A3A, ARP3, PHRBN, bK150C2.1, DNA dC->dU-editing enzyme APOBEC-3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, phorbolin-1, probable DNA dC->dU-editing enzyme APOBEC-3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31941
  • Protein Name
    DNA dC->dU-editing enzyme APOBEC-3A
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of APOBEC3A using anti-APOBEC3A antibody (A01183-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3A antigen affinity purified polyclonal antibody (Catalog # A01183-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3A at approximately 23 kDa. The expected band size for APOBEC3A is at 23 kDa.
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