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Immunohistochemical staining of human colon shows moderate to strong positivity in plasma in blood vessels.
Immunohistochemical staining of human colon shows moderate to strong positivity in plasma in blood vessels.
Immunohistochemical staining of human colon shows moderate to strong positivity in plasma in blood vessels.

Anti-APOL1 Antibody

HPA018885
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAPOL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-APOL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8542
  • Target name
    APOL1
  • Target description
    apolipoprotein L1
  • Target synonyms
    APO-L, APOL, APOL-I, FSGS4, apolipoprotein L1, apolipoprotein L 1, apolipoprotein L1-B3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14791
  • Protein Name
    Apolipoprotein L1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of APOL1 using anti-APOL1 antibody (A01841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOL1 antigen affinity purified polyclonal antibody (Catalog # A01841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOL1 at approximately 37KD. The expected band size for APOL1 is at 44KD.
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