Figure 1. Western blot analysis of Aqp2 using anti-Aqp2 antibody (A00935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aqp2 antigen affinity purified polyclonal antibody (Catalog # A00935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aqp2 at approximately 26 kDa. The expected band size for Aqp2 is at 30 kDa.
Figure 1. Western blot analysis of Aqp2 using anti-Aqp2 antibody (A00935). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aqp2 antigen affinity purified polyclonal antibody (Catalog # A00935) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aqp2 at approximately 26 kDa. The expected band size for Aqp2 is at 30 kDa.
Anti-Aqp2 Antibody Picoband(r)
A00935-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetAqp2
Overview
- SupplierBoster Bio
- Product NameAnti-Aqp2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID11827
- Target nameAqp2
- Target descriptionaquaporin 2
- Target synonymsAQP-CD, WCH-CD, cph, jpk, aquaporin-2, ADH water channel, AQP-2, aquaporin-CD, collecting duct water channel protein, water channel protein for renal collecting duct
- HostRabbit
- IsotypeIgG
- Protein IDP56402
- Protein NameAquaporin-2
- Scientific DescriptionBoster Bio Anti-Aqp2 Antibody Picoband® catalog # A00935. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
