Anti-Arginase 1 [mAb5]

Absolute Antibody

Anti-Arginase 1 [mAb5]

7

The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 +/- 1.7 nM (Palte et al., 2021; PMID: 34326456).

Electron Microscopy, Neutralisation/Blocking

Cryo-electron microscopy; Inhibition

Research Use Only

Monoclonal

mAb5

ARG1

arginase 1

arginase-1, arginase, liver, liver-type arginase, type I arginase

Mouse

IgG1

Arginase-1

Human

The antibody was generated by immunizing mice with recombinant arginase 1.

-20°C,2°C to 8°C

12352203