Bio-Connect
Anti-ARHGEF5 Antibody

Anti-ARHGEF5 Antibody

A96594
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ARHGEF5 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to ARHGEF5.
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetARHGEF5
  • SizePrice
Product group Antibodies
Assay Genie
TargetARHGEF5
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetARHGEF5
  • SizePrice
Product group Antibodies
Cusabio
ARHGEF5 AntibodyCSB-PA060165
ApplicationsWestern Blot, ELISA
TargetARHGEF5
  • SizePrice
Figure 1. Western blot analysis of ARHGEF5 using anti-ARHGEF5 antibody (A01487-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF5 antigen affinity purified polyclonal antibody (Catalog # A01487-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF5 at approximately 250 kDa. The expected band size for ARHGEF5 is at 177 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetARHGEF5
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetARHGEF5
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetARHGEF5
  • SizePrice
Product group Antibodies
Abbexa
GEF5 AntibodyABX432739
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetARHGEF5
  • SizePrice
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
TargetARHGEF5
  • SizePrice