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Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Anti-ARID5A Antibody

HPA023879
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARID5A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ARID5A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10865
  • Target name
    ARID5A
  • Target description
    AT-rich interaction domain 5A
  • Target synonyms
    MRF-1, MRF1, RP11-363D14, AT-rich interactive domain-containing protein 5A, ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), RFVG5814, modulator recognition factor 1, modulator recognition factor I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03989
  • Protein Name
    AT-rich interactive domain-containing protein 5A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (A14678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # A14678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD.
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