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Figure 1. Western blot analysis of ARL6IP6 using anti-ARL6IP6 antibody (A14815). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL6IP6 antigen affinity purified polyclonal antibody (Catalog # A14815) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL6IP6 at approximately 22 kDa. The expected band size for ARL6IP6 is at 22 kDa.
Figure 1. Western blot analysis of ARL6IP6 using anti-ARL6IP6 antibody (A14815). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL6IP6 antigen affinity purified polyclonal antibody (Catalog # A14815) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL6IP6 at approximately 22 kDa. The expected band size for ARL6IP6 is at 22 kDa.
Figure 1. Western blot analysis of ARL6IP6 using anti-ARL6IP6 antibody (A14815). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL6IP6 antigen affinity purified polyclonal antibody (Catalog # A14815) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL6IP6 at approximately 22 kDa. The expected band size for ARL6IP6 is at 22 kDa.

Anti-ARL6IP6 Antibody Picoband(r)

A14815-IFLUOR647
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARL6IP6
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-ARL6IP6 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID151188
  • Target name
    ARL6IP6
  • Target description
    ADP ribosylation factor like GTPase 6 interacting protein 6
  • Target synonyms
    ADP-ribosylation factor GTPase 6 interacting protein 6; ADP-ribosylation factor-like 6 interacting protein 6; ADP-ribosylation factor-like protein 6-interacting protein 6; ADP-ribosylation-like factor 6 interacting protein 6; AIP6; AIP-6; ARL-6-interacting protein 6; PFAAP1; phosphonoformate immuno-associated protein 1; regulated by phosphonoformate
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6S5
  • Protein Name
    ADP-ribosylation factor-like protein 6-interacting protein 6
  • Scientific Description
    Boster Bio Anti-ARL6IP6 Antibody Picoband® catalog # A14815. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203