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Anti-ASC Antibody

ORB1149207
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
TargetPYCARD
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-ASC Antibody
  • Delivery Days Customer
    10
  • Applications
    Western Blot
  • Applications Supplier
    Immunoblotting: use at 1ug/ml. Immunocytochemistry: use at 5ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate of HL60 cells. WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29108
  • Target name
    PYCARD
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain-containing protein 5; target of methylation-induced silencing 1; TMS; TMS1; TMS-1
  • Host
    Rabbit
  • Protein IDQ9ULZ3
  • Protein Name
    Apoptosis-associated speck-like protein containing a CARD
  • Scientific Description
    Anti-ASC Rabbit Polyclonal Antibody
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.
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