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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Anti-ASIC2 Antibody

HPA052112
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetASIC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ASIC2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID40
  • Target name
    ASIC2
  • Target description
    acid sensing ion channel subunit 2
  • Target synonyms
    ACCN, ACCN1, ASIC2a, BNC1, BNaC1, MDEG, hBNaC1, acid-sensing ion channel 2, acid sensing (proton gated) ion channel 2, amiloride-sensitive cation channel 1, neuronal, brain Na+ channel-1, brain sodium channel 1, mammalian degenerin homolog, neuronal amiloride-sensitive cation channel 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16515
  • Protein Name
    Acid-sensing ion channel 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASIC2 using anti-ASIC2 antibody (A04055-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat ovarian tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse ovarian tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC2 antigen affinity purified polyclonal antibody (Catalog # A04055-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC2 at approximately 58KD. The expected band size for ASIC2 is at 58KD.
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TargetASIC2
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TargetASIC2
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