Anti-Astrovirus 2 [PL-2]

Absolute Antibody

Anti-Astrovirus 2 [PL-2]

9

The antibody binds to the surface of the HAstV-2 virion, which is formed by the virus capsid protein. The antibody binds to a conformation-dependent quaternary epitope on each side of the dimeric capsid spike. Strains of HAstV-2 (HAstV-2-Oxford, -RIVMa, -

The original antibody (IgG2a) was used for immunofluorescence staining of H-Ast2-infected LLCMK2 cells. The antibody neutralizes HAstV serotype 2 (Sanchez-Fauquier et al., 1994; PMID: 7514320). The original antibody (IgG2a) and the scFv fragment bound to wheat germ extract containing recombinant HAstV-2 capsid protein, as confirmed by ELISA analysis. The crystal structure of the Fab fragment was determined. The scFv fragment was used to immunoprecipitate HAstV-2 capsid protein. This antibody could potentially be developed as a therapeutic and diagnostic agent (Bogdanoff et al., 2016; PMID: 27213181). The specificity of the antibody and scFv fragment for the HAstV-2 CP spike were confirmed by ELISA. The binding affinity of the Fab and scFv fragments towards the spike protein was found to be nearly identical (KD of 1.8 nM) using surface plasmon resonance. The structure of the HAstV capsid spike domain bound to the neutralizing scFv fragment was determined. The antibody blocked the attachment of recombinant HAstV-2 CP spike to Caco-2 cells supporting the hypothesis the antibody neutralizes HAstV by blocking virus attachment to cells (Bogdanoff et al., 2016; PMID: 27807234).

ImmunoPrecipitation, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

PL-2

Human

IgG1

Capsid polyprotein VP90

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203