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Immunohistochemistry analysis in human stomach and pancreas tissues using Anti-ATP4A antibody. Corresponding ATP4A RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human stomach and pancreas tissues using Anti-ATP4A antibody. Corresponding ATP4A RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human stomach and pancreas tissues using Anti-ATP4A antibody. Corresponding ATP4A RNA-seq data are presented for the same tissues.

Anti-ATP4A Antibody

HPA076684
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetATP4A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATP4A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID495
  • Target name
    ATP4A
  • Target description
    ATPase H+/K+ transporting subunit alpha
  • Target synonyms
    ATP6A, potassium-transporting ATPase alpha chain 1, ATPase H+/K+ transporting alpha subunit, ATPase, H+/K+ exchanging, alpha polypeptide, ATPase, H+/K+ transporting, alpha polypeptide, epididymis secretory sperm binding protein, gastric H+/K+ ATPase alpha subunit, gastric H,K-ATPase catalytic subunit, gastric hydrogen-potassium ATPase, proton pump
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20648
  • Protein Name
    Potassium-transporting ATPase alpha chain 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ATP4A using anti-ATP4A antibody (A08198-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP4A antigen affinity purified polyclonal antibody (Catalog # A08198-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP4A at approximately 130 kDa. The expected band size for ATP4A is at 114 kDa.
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Immunohistochemistry analysis in human stomach and pancreas tissues using Anti-ATP4A antibody. Corresponding ATP4A RNA-seq data are presented for the same tissues.
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